An improved tissue culture assay. II. Cytotoxicity studies with antibiotics, chemicals, and solvents.

In the preceding publication of this series (10), a simplified tissue culture assay was described. The cytotoxic activity of various nucleic acid antagonists, antibiotics, amino acid and vitamin antagonists, respiratory poisons, miscellaneous chemicals, solvents, heavy metals, detergents, and fermentation beers were determined with the new into sterile bottles and dissolved in water (sterile), dimethylformamide (nonsterile), or ethanol (nonsterile). Solvents were diluted in sterile water to a final concentration of less than 1000 gg/ml. For preliminary screening, test compounds were assayed at 500, 100, 20, 4, and 0.8 ~g/ml with one assay tube per concentration. Agents with an ID60